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janus automated liquid handling workstation  (Revvity)


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    Structured Review

    Revvity janus automated liquid handling workstation
    Janus Automated Liquid Handling Workstation, supplied by Revvity, used in various techniques. Bioz Stars score: 94/100, based on 1681 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/janus+automated+workstation/JANUS+liquid+handling+workstations/pmc13112130-160-50-55
    Average 94 stars, based on 1681 article reviews
    janus automated liquid handling workstation - by Bioz Stars, 2026-09
    94/100 stars

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    Sequencing:

    Article Title: Interkingdom interaction: the soil isopod Porcellio scaber stimulates the methane-driven bacterial and fungal interaction
    Article Snippet: Next, the amplicons were purified using the MagSi-NGS PREP Plus Magnetic beads (Steinbrenner Laborsysteme GmbH, Wiesenbach, Germany), as described by the manufacturer. .. Normalization of the amplicons (equimolar amounts, 133 ng per sample) was performed using the Janus Automated Workstation (Perkin Elmer, Waltham, Massachusetts, USA) for library preparation and sequencing using Illumina MiSeq version 3 chemistry (paired-end, 600 cycles). ..

    SYBR Green Assay:

    Article Title: Hexokinase regulates Mondo-mediated longevity via the PPP and organellar dynamics
    Article Snippet: The concentration and purity of the RNA samples were quantified using the NanoPhotometer NP80 spectrophotometer (Implen). cDNA was prepared after extracting total RNA from young adult worms or cell extracts using the iScript cDNA Synthesis Kit (BioRad) according to the manufacturer’s instructions. .. Primers, cDNA, and Power SYBR Green Master Mix (Applied Biosystems) were transferred to a 384-well plate using the JANUS automated workstation (PerkinElmer) with four technical replicates per genotype/gene. .. The reaction was quantified using the ViiA 7 Real-Time PCR system machine (Applied Biosystems). cdc-42 was used as an endogenous control for the quantification.

    Concentration Assay:

    Article Title: Monensin as potential drug for treatment of SLeX-positive tumors
    Article Snippet: COLO205 cells were seeded in 384-well plates (CellCarrier-384 Ultra, PerkinElmer) at a density of 1 × 10 5 cells/mL in RPMI medium supplemented with 10% FBS and allowed to adhere overnight. .. Two distinct libraries totalizing 7,836 chemical compounds (1,248 compounds from the Prestwick Chemical Library®, and 6,588 from DIVERSet® library) were then transferred, one compound per well, using a pintool (V&P Scientific) coupled to a MDT head of a JANUS Automated Workstation (PerkinElmer) for a final concentration of 4 μM in DMSO. .. Cells were incubated for 72 h, after which they were fixed with formaldehyde to a final concentration of 4% (Sigma Aldrich).

    Subculturing Assay:

    Article Title: A high-throughput cytotoxicity screening platform reveals agr-independent mutations in bacteraemia-associated Staphylococcus aureus that promote intracellular persistence
    Article Snippet: .. After subculturing strains twice from −80°C glycerol stock, DNA was extracted using the Janus automated workstation (PerkinElmer) or manual extraction kits (Invitrogen PureLink genomic DNA kit or the Sigma GenElute kit). .. Normalised DNA (at a concentration of 0.2 ng/ml) was prepared for sequencing using Nextera XT DNA (Illumina) and sequencing was performed on Illumina MiSeq and NextSeq platforms.

    Article Title: A high-throughput cytotoxicity screening platform reveals agr -independent mutations in bacteraemia-associated Staphylococcus aureus that promote intracellular persistence
    Article Snippet: .. After subculturing strains twice from −80°C glycerol stock, DNA was extracted using the Janus automated workstation (PerkinElmer) or manual extraction kits (Invitrogen PureLink genomic DNA kit or the Sigma GenElute kit). .. Normalised DNA (at a concentration of 0.2 ng/ml) was prepared for sequencing using Nextera XT DNA (Illumina) and sequencing was performed on Illumina MiSeq and NextSeq platforms.

    Extraction:

    Article Title: A high-throughput cytotoxicity screening platform reveals agr-independent mutations in bacteraemia-associated Staphylococcus aureus that promote intracellular persistence
    Article Snippet: .. After subculturing strains twice from −80°C glycerol stock, DNA was extracted using the Janus automated workstation (PerkinElmer) or manual extraction kits (Invitrogen PureLink genomic DNA kit or the Sigma GenElute kit). .. Normalised DNA (at a concentration of 0.2 ng/ml) was prepared for sequencing using Nextera XT DNA (Illumina) and sequencing was performed on Illumina MiSeq and NextSeq platforms.

    Article Title: A high-throughput cytotoxicity screening platform reveals agr -independent mutations in bacteraemia-associated Staphylococcus aureus that promote intracellular persistence
    Article Snippet: .. After subculturing strains twice from −80°C glycerol stock, DNA was extracted using the Janus automated workstation (PerkinElmer) or manual extraction kits (Invitrogen PureLink genomic DNA kit or the Sigma GenElute kit). .. Normalised DNA (at a concentration of 0.2 ng/ml) was prepared for sequencing using Nextera XT DNA (Illumina) and sequencing was performed on Illumina MiSeq and NextSeq platforms.



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    Pipetting analysis of microgels, cells and scaffolds with the automated liquid handling workstation <t>JANUS</t> <t>G3.</t> a , Scaffold generation in low-volume 384 well plates on day 0, 1 and 2 with different ratios and amounts of cells and microgels. Depending on the initial ratios and amounts used scaffolds of differing sizes were achieved. Scale bar: 500 µm. b , Transfer of scaffolds between wells using the automated pipetting on day 5. Successful transfer of scaffolds was observed resulting in removal of cell debris for further culture. Scale bar: 500 µm.
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    Image Search Results


    Pipetting analysis of microgels, cells and scaffolds with the automated liquid handling workstation JANUS G3. a , Scaffold generation in low-volume 384 well plates on day 0, 1 and 2 with different ratios and amounts of cells and microgels. Depending on the initial ratios and amounts used scaffolds of differing sizes were achieved. Scale bar: 500 µm. b , Transfer of scaffolds between wells using the automated pipetting on day 5. Successful transfer of scaffolds was observed resulting in removal of cell debris for further culture. Scale bar: 500 µm.

    Journal: bioRxiv

    Article Title: Microgels enable iPSCs to assemble, expand, and differentiate into organoids – from sizable to high throughput

    doi: 10.1101/2025.10.20.683388

    Figure Lengend Snippet: Pipetting analysis of microgels, cells and scaffolds with the automated liquid handling workstation JANUS G3. a , Scaffold generation in low-volume 384 well plates on day 0, 1 and 2 with different ratios and amounts of cells and microgels. Depending on the initial ratios and amounts used scaffolds of differing sizes were achieved. Scale bar: 500 µm. b , Transfer of scaffolds between wells using the automated pipetting on day 5. Successful transfer of scaffolds was observed resulting in removal of cell debris for further culture. Scale bar: 500 µm.

    Article Snippet: JANUS G3 automated liquid handling workstations (Revvity, USA) was used for automated production of scaffolds in high-throughput format.

    Techniques: